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goat anti human nectin4 polyclonal antibody  (R&D Systems)


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    R&D Systems goat anti human nectin4 polyclonal antibody
    Fig. 1. Infection of the parental, <t>nectin4-expressing,</t> and SLAM-expressing Vero cells with wild-type CDV strains. (A) Vero/hNectin4 (left panel) and Vero/dNectin4 (right panel) cells were stained with a goat anti-human nectin4 polyclonal antibody (gray empty profile) or a control goat IgG (filled black profile), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) Vero, Vero/ hNectin4, Vero/dNectin4, Vero/hSLAM, and Vero.DogSLAMtag cells were infected with wild-type CDV strains (Ac96I-VDS, 82Con, 55L, M24Cr, and Th12) or mock- infected. At 48 h (Vero, Vero/hNectin4, Vero/dNectin4, and Vero/hSLAM) or 24 h (Vero.DogSLAMtag) post-infection, the cells were stained with the Giemsa solu- tion, and observed under a phase-contrast microscope. (C) Replication kinetics of Ac96I. Vero/hNectin4, Vero/dNectin4, and parental Vero cells were infected with Ac96I at a MOI of 0.01. At various time intervals post-infection, the virus titers were determined by plaque assays.
    Goat Anti Human Nectin4 Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+human+nectin4+polyclonal+antibody/Human+Nectin-4+Antibody/pm23174504-156-10-15
    Average 94 stars, based on 33 article reviews
    goat anti human nectin4 polyclonal antibody - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor."

    Article Title: Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor.

    Journal: Virology

    doi: 10.1016/j.virol.2012.10.033

    Fig. 1. Infection of the parental, nectin4-expressing, and SLAM-expressing Vero cells with wild-type CDV strains. (A) Vero/hNectin4 (left panel) and Vero/dNectin4 (right panel) cells were stained with a goat anti-human nectin4 polyclonal antibody (gray empty profile) or a control goat IgG (filled black profile), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) Vero, Vero/ hNectin4, Vero/dNectin4, Vero/hSLAM, and Vero.DogSLAMtag cells were infected with wild-type CDV strains (Ac96I-VDS, 82Con, 55L, M24Cr, and Th12) or mock- infected. At 48 h (Vero, Vero/hNectin4, Vero/dNectin4, and Vero/hSLAM) or 24 h (Vero.DogSLAMtag) post-infection, the cells were stained with the Giemsa solu- tion, and observed under a phase-contrast microscope. (C) Replication kinetics of Ac96I. Vero/hNectin4, Vero/dNectin4, and parental Vero cells were infected with Ac96I at a MOI of 0.01. At various time intervals post-infection, the virus titers were determined by plaque assays.
    Figure Legend Snippet: Fig. 1. Infection of the parental, nectin4-expressing, and SLAM-expressing Vero cells with wild-type CDV strains. (A) Vero/hNectin4 (left panel) and Vero/dNectin4 (right panel) cells were stained with a goat anti-human nectin4 polyclonal antibody (gray empty profile) or a control goat IgG (filled black profile), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) Vero, Vero/ hNectin4, Vero/dNectin4, Vero/hSLAM, and Vero.DogSLAMtag cells were infected with wild-type CDV strains (Ac96I-VDS, 82Con, 55L, M24Cr, and Th12) or mock- infected. At 48 h (Vero, Vero/hNectin4, Vero/dNectin4, and Vero/hSLAM) or 24 h (Vero.DogSLAMtag) post-infection, the cells were stained with the Giemsa solu- tion, and observed under a phase-contrast microscope. (C) Replication kinetics of Ac96I. Vero/hNectin4, Vero/dNectin4, and parental Vero cells were infected with Ac96I at a MOI of 0.01. At various time intervals post-infection, the virus titers were determined by plaque assays.

    Techniques Used: Infection, Expressing, Staining, Control, Microscopy, Virus

    Fig. 2. Amino acid sequence comparison of the V domains of human, dog, and mouse nectin4. Dots indicate identical residues to those of human nectin4.
    Figure Legend Snippet: Fig. 2. Amino acid sequence comparison of the V domains of human, dog, and mouse nectin4. Dots indicate identical residues to those of human nectin4.

    Techniques Used: Sequencing, Comparison

    Fig. 4. Replication kinetics in NCI-H358 cells. (A) NCI-H358 cells were stained with a goat anti-human nectin4 polyclonal antibody (gray empty profile) or a control goat IgG (filled black profile), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) NCI-H358 cells were infected with the Ac96I-VDS, 82Con, 55 L, M24Cr, or Th12 CDV strains at a MOI of 0.01. At 5 days post-infection, the virus titers were determined by plaque assays. (C, D) NCI-H358 (C) and II-18 (D) cells were infected with Ac96I-VDS or Ac96I-H358 at a MOI of 0.01. At 1, 3, 5, and 7 days post-infection, the virus titers were determined by plaque assays.
    Figure Legend Snippet: Fig. 4. Replication kinetics in NCI-H358 cells. (A) NCI-H358 cells were stained with a goat anti-human nectin4 polyclonal antibody (gray empty profile) or a control goat IgG (filled black profile), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) NCI-H358 cells were infected with the Ac96I-VDS, 82Con, 55 L, M24Cr, or Th12 CDV strains at a MOI of 0.01. At 5 days post-infection, the virus titers were determined by plaque assays. (C, D) NCI-H358 (C) and II-18 (D) cells were infected with Ac96I-VDS or Ac96I-H358 at a MOI of 0.01. At 1, 3, 5, and 7 days post-infection, the virus titers were determined by plaque assays.

    Techniques Used: Staining, Control, Infection, Virus

    Related Articles

    Expressing:

    Article Title: Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor.
    Article Snippet: .. The cell surface expression of nectin4 was analyzed using a goat anti-human nectin4 polyclonal antibody (R&D Systems, Minneapolis, MN) as the primary antibody and Alexa Fluor 488-conjugated anti-goat IgG (Molecular probes, Eugene, Oregon) as the secondary antibody. .. Normal goat IgG (PEPROTECH, Rocky Hill, NJ) was used as a control.

    Infection:

    Article Title: Nectin4 Is an Epithelial Cell Receptor for Canine Distemper Virus and Involved in Neurovirulence
    Article Snippet: .. All Rights Reserved. doi:10.1128/JVI.00824-12 September 2012 Volume 86 Number 18 Journal of Virology p. 10207–10210 jvi.asm.org 10207 on July 2, 2015 by U N IV O F C O N N E C T IC U T http://jvi.asm .org/ D ow nloaded from FIG 1 Infection of Vero/dNectin4 cells with CDV. (A) Vero/dNectin4 (gray empty profile) and parental Vero (filled black profile) cells were stained with a goat anti-human nectin4 polyclonal antibody (R&D Systems), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) Vero/dNectin4 (right panel) and parental Vero (left panel) cells were infected with the Ac96I strain. ..

    Article Title: Nectin4 Is an Epithelial Cell Receptor for Canine Distemper Virus and Involved in Neurovirulence
    Article Snippet: .. These findings indicate that dog nectin4 functions as a CDV receptor, similar to the case with MV ( 13 , 14 ). fig ft0 fig mode=article f1 Fig 1 caption a4 Infection of Vero/dNectin4 cells with CDV. (A) Vero/dNectin4 (gray empty profile) and parental Vero (filled black profile) cells were stained with a goat anti-human nectin4 polyclonal antibody (R&D Systems), followed by staining with Alexa Fluor ... ..

    Staining:

    Article Title: Nectin4 Is an Epithelial Cell Receptor for Canine Distemper Virus and Involved in Neurovirulence
    Article Snippet: .. All Rights Reserved. doi:10.1128/JVI.00824-12 September 2012 Volume 86 Number 18 Journal of Virology p. 10207–10210 jvi.asm.org 10207 on July 2, 2015 by U N IV O F C O N N E C T IC U T http://jvi.asm .org/ D ow nloaded from FIG 1 Infection of Vero/dNectin4 cells with CDV. (A) Vero/dNectin4 (gray empty profile) and parental Vero (filled black profile) cells were stained with a goat anti-human nectin4 polyclonal antibody (R&D Systems), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) Vero/dNectin4 (right panel) and parental Vero (left panel) cells were infected with the Ac96I strain. ..

    Article Title: Nectin4 Is an Epithelial Cell Receptor for Canine Distemper Virus and Involved in Neurovirulence
    Article Snippet: .. These findings indicate that dog nectin4 functions as a CDV receptor, similar to the case with MV ( 13 , 14 ). fig ft0 fig mode=article f1 Fig 1 caption a4 Infection of Vero/dNectin4 cells with CDV. (A) Vero/dNectin4 (gray empty profile) and parental Vero (filled black profile) cells were stained with a goat anti-human nectin4 polyclonal antibody (R&D Systems), followed by staining with Alexa Fluor ... ..



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    goat anti human nectin4 polyclonal antibody  (R&D Systems)
    94
    R&D Systems goat anti human nectin4 polyclonal antibody
    Fig. 1. Infection of the parental, <t>nectin4-expressing,</t> and SLAM-expressing Vero cells with wild-type CDV strains. (A) Vero/hNectin4 (left panel) and Vero/dNectin4 (right panel) cells were stained with a goat anti-human nectin4 polyclonal antibody (gray empty profile) or a control goat IgG (filled black profile), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) Vero, Vero/ hNectin4, Vero/dNectin4, Vero/hSLAM, and Vero.DogSLAMtag cells were infected with wild-type CDV strains (Ac96I-VDS, 82Con, 55L, M24Cr, and Th12) or mock- infected. At 48 h (Vero, Vero/hNectin4, Vero/dNectin4, and Vero/hSLAM) or 24 h (Vero.DogSLAMtag) post-infection, the cells were stained with the Giemsa solu- tion, and observed under a phase-contrast microscope. (C) Replication kinetics of Ac96I. Vero/hNectin4, Vero/dNectin4, and parental Vero cells were infected with Ac96I at a MOI of 0.01. At various time intervals post-infection, the virus titers were determined by plaque assays.
    Goat Anti Human Nectin4 Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+human+nectin4+polyclonal+antibody/Human+Nectin-4+Antibody/pm23174504-156-10-15
    Average 94 stars, based on 1 article reviews
    goat anti human nectin4 polyclonal antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 1. Infection of the parental, nectin4-expressing, and SLAM-expressing Vero cells with wild-type CDV strains. (A) Vero/hNectin4 (left panel) and Vero/dNectin4 (right panel) cells were stained with a goat anti-human nectin4 polyclonal antibody (gray empty profile) or a control goat IgG (filled black profile), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) Vero, Vero/ hNectin4, Vero/dNectin4, Vero/hSLAM, and Vero.DogSLAMtag cells were infected with wild-type CDV strains (Ac96I-VDS, 82Con, 55L, M24Cr, and Th12) or mock- infected. At 48 h (Vero, Vero/hNectin4, Vero/dNectin4, and Vero/hSLAM) or 24 h (Vero.DogSLAMtag) post-infection, the cells were stained with the Giemsa solu- tion, and observed under a phase-contrast microscope. (C) Replication kinetics of Ac96I. Vero/hNectin4, Vero/dNectin4, and parental Vero cells were infected with Ac96I at a MOI of 0.01. At various time intervals post-infection, the virus titers were determined by plaque assays.

    Journal: Virology

    Article Title: Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor.

    doi: 10.1016/j.virol.2012.10.033

    Figure Lengend Snippet: Fig. 1. Infection of the parental, nectin4-expressing, and SLAM-expressing Vero cells with wild-type CDV strains. (A) Vero/hNectin4 (left panel) and Vero/dNectin4 (right panel) cells were stained with a goat anti-human nectin4 polyclonal antibody (gray empty profile) or a control goat IgG (filled black profile), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) Vero, Vero/ hNectin4, Vero/dNectin4, Vero/hSLAM, and Vero.DogSLAMtag cells were infected with wild-type CDV strains (Ac96I-VDS, 82Con, 55L, M24Cr, and Th12) or mock- infected. At 48 h (Vero, Vero/hNectin4, Vero/dNectin4, and Vero/hSLAM) or 24 h (Vero.DogSLAMtag) post-infection, the cells were stained with the Giemsa solu- tion, and observed under a phase-contrast microscope. (C) Replication kinetics of Ac96I. Vero/hNectin4, Vero/dNectin4, and parental Vero cells were infected with Ac96I at a MOI of 0.01. At various time intervals post-infection, the virus titers were determined by plaque assays.

    Article Snippet: The cell surface expression of nectin4 was analyzed using a goat anti-human nectin4 polyclonal antibody (R&D Systems, Minneapolis, MN) as the primary antibody and Alexa Fluor 488-conjugated anti-goat IgG (Molecular probes, Eugene, Oregon) as the secondary antibody.

    Techniques: Infection, Expressing, Staining, Control, Microscopy, Virus

    Fig. 2. Amino acid sequence comparison of the V domains of human, dog, and mouse nectin4. Dots indicate identical residues to those of human nectin4.

    Journal: Virology

    Article Title: Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor.

    doi: 10.1016/j.virol.2012.10.033

    Figure Lengend Snippet: Fig. 2. Amino acid sequence comparison of the V domains of human, dog, and mouse nectin4. Dots indicate identical residues to those of human nectin4.

    Article Snippet: The cell surface expression of nectin4 was analyzed using a goat anti-human nectin4 polyclonal antibody (R&D Systems, Minneapolis, MN) as the primary antibody and Alexa Fluor 488-conjugated anti-goat IgG (Molecular probes, Eugene, Oregon) as the secondary antibody.

    Techniques: Sequencing, Comparison

    Fig. 4. Replication kinetics in NCI-H358 cells. (A) NCI-H358 cells were stained with a goat anti-human nectin4 polyclonal antibody (gray empty profile) or a control goat IgG (filled black profile), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) NCI-H358 cells were infected with the Ac96I-VDS, 82Con, 55 L, M24Cr, or Th12 CDV strains at a MOI of 0.01. At 5 days post-infection, the virus titers were determined by plaque assays. (C, D) NCI-H358 (C) and II-18 (D) cells were infected with Ac96I-VDS or Ac96I-H358 at a MOI of 0.01. At 1, 3, 5, and 7 days post-infection, the virus titers were determined by plaque assays.

    Journal: Virology

    Article Title: Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor.

    doi: 10.1016/j.virol.2012.10.033

    Figure Lengend Snippet: Fig. 4. Replication kinetics in NCI-H358 cells. (A) NCI-H358 cells were stained with a goat anti-human nectin4 polyclonal antibody (gray empty profile) or a control goat IgG (filled black profile), followed by staining with Alexa Fluor 488-conjugated anti-goat IgG. (B) NCI-H358 cells were infected with the Ac96I-VDS, 82Con, 55 L, M24Cr, or Th12 CDV strains at a MOI of 0.01. At 5 days post-infection, the virus titers were determined by plaque assays. (C, D) NCI-H358 (C) and II-18 (D) cells were infected with Ac96I-VDS or Ac96I-H358 at a MOI of 0.01. At 1, 3, 5, and 7 days post-infection, the virus titers were determined by plaque assays.

    Article Snippet: The cell surface expression of nectin4 was analyzed using a goat anti-human nectin4 polyclonal antibody (R&D Systems, Minneapolis, MN) as the primary antibody and Alexa Fluor 488-conjugated anti-goat IgG (Molecular probes, Eugene, Oregon) as the secondary antibody.

    Techniques: Staining, Control, Infection, Virus